![]() Store the activated stain at room temperature. Mix the activated GelCode Blue Safe Protein Stain immediately before use by gently inverting or tipping and swirling the bottle several times. Dissolve the crystals by mixing end-over-end for one minute, then allow it to remain at room temperature for five minutes before use. Wash the gel by microwaving for 90 seconds in 100 ml of ultrapure water. Add the entire contents of the activator crystals bottle into the GelCode Blue Safe Protein Stain bottle. For an unstained gel, if the transfer has been successful the Precision Plus Protein Kaleidoscope prestained protein standards will be visible on the membrane. ![]() For proper protein staining, the activator crystals must be dissolved in the staining reagent before use. For a stained gel, if the transfer has been successful, the blue stained proteins will show up on the membrane and no blue stained proteins should be visible on the gel. Department of Transportation (DOT) shipping requirements.
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